Phenotypic… | Phenotypic and molecular characterisation of cytotoxic Escherichia coli and their bacteriophages

The group continues the detailed genetic characterisation of E. coli O157 and Shiga toxin (Stx) producing Shigella sonnei strains identified in a previous project. They also plan the intestinal metagenomic study of livestock.

Project leader: 

On the basis of earlier Széchenyi and OTKA grants (K37890, K81252), as well as NKFI grant applications (K119716, K119692) they plan to identify novel clones/strains of Shiga toxigenic E. coli (STEC) representing O157 and non-O157 serogroups. They aim to determine and annotate their genomic sequences, deposit them in GenBank and publish results. An additional important task is determining the host spectrum and genotyping of bacteriophages isolated up to date and also those to be isolated in the future. These studies are necessary to assess the evolutionary role and diagnostic potential of these phages. They plan to isolate and characterise further cytotoxic E. coli (strains producing cytolethal distending toxin (CDT), Stx etc.) from cattle. Another plan is to establish an enteric strain collection from sick and healthy poultry, to explore the pathogenic and zoonotic potential of the isolates, and as an implementation of a new methodology, they also plan metagenomic studies on the intestinal microbiota in poultry.

Researcher(s) of IVMR on the Project: