OTKA K 105635 | Molecular genetics and ecology of Salmonella Genomic Island 1 (SGI1): mobility, spread and pathogenetic significance

An increase of public health and veterinary significance of some strains of Salmonella Typhimurium in the last 20 years is partly explained by the emergence of a multiresistance genetic island (SGI1). The subtask of VMRI is focusing on the significance of SGI1 in pathogenetic and spreading attributes of S. Typhimurium.

Period of the research activity: 

2013-2017

Project leader: 

Kiss, János researcher (NAIK, Agricultural Biotechnology Center, Gödöllő, Hungary)

Financial support: 

OTKA K 105635

SGI1 has been first described as a genomic island of 43 kb, encoding multidrug resistance, in isolates of S. Typhimurium. DT104 and this was confirmed by the early Hungarian studies on food producing animals (Fig. 1). Specific gene functions and conditions for mobility of SGI1 have been first studied by French and Canadian as well as by Hungarian groups. The research in Hungary is accomplished in cooperation of J. Kiss’s group (NAIK-ABC) and this IVMR team in frame of the OTKA K-105635 Project. The main task of the IVMR team is to study some details of the in vivo and in vitro horizontal transfer of SGI1 and of its pathogenetic potential.
 
The in vivo transfer of the SGI1 is studied by using appropriately markered donor strains carrying SGI1 and a helper plasmid. The transfer of SGI1 into E. coli and the microbiome of the chicken intestine is monitored by approaches specific to E. coli and by metagenomics approaches. The transfer and its molecular mechanisms including the role of the helper plasmids are examined by the Project leader’s group. Pathogenic significance of SGI1 is being studied by the use of isogenic mutants.
Researcher(s) of IVMR on the Project: 
Further participants and collaborators on the Project: 

Olasz, Ferenc senior researcher, Murányi, Gábor PhD-student, Hegyi, Anna PhD-student, Szabó, Mónika researcher, Nagy, István PhD-student (all in NAIK, Agricultural Biotechnology Center, Gödöllő, Hungary)